Please be able to do a 3-point testcross for Exam I. If you need further help, re-read pages 154 - 156 in your text. In Drosophila: y = yellow body color / y+ = gray v = vermilion eye color / v+ = red sn = singed bristles / sn+ = straight bristles test cross performed, with resulting progeny: yellow, vermilion, singed 53 yellow, vermilion 108 yellow, singed 331 yellow 5 vermilion, singed 3 vermilion 342 singed 95 wildtype 63 TOTAL 1000 Identify the parental (P) phenotypes: P chromosomes yellow, singed 331 y + sn vermilion 342 + v + Identify the double recombinant (2xR) phenotypes: 2xR chromosomes yellow 5 y + + vermilion, singed 3 + v sn what is the gene order? y sn v OR v sn y because if you compare the parental chromosomes to the 2xR chromosomes, you will see that the gene that has the opposite orientation is the sn locus. How far apart are the genes in map units? between y and sn: 95 + 108 + 5 + 3 = 211 211/1000 = 0.211 x 100 = 21.1 map units between sn and v 63 + 53 + 5 + 3 = 124 124/1000 = 0.124 x 100 = 12.4 map units What is the coefficient of coincidence and interference for this region of the chromosome? the observed frequency of double crossovers (from the data above) is 5 + 3 = 8/1000, or 0.008 probability of a crossover between y and sn is 0.211 probability of a crossover between sn and v is 0.124 therefore, the expected frequency of 2 crossovers between y and v is (0.211) x (0.124) = 0.026 the coefficient of coincidence is the observed frequency divided by the expected frequency: 0.008/0.026 = 0.307 the interference is 1 - the coefficient of coincidence: 1 - 0.307 = 0.693